Book:Next-Generation Sequencing: A Brief Introduction
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Next-Generation Sequencing: A Brief Introduction
The goal of this short volume is to act as "a collective lab book that explains the key concepts and describes best practices in NGS" or next generation sequencing. The content originates from Wikibooks and is made available under the Creative Commons Attribution-ShareAlike 3.0 Unported license.
- 1. What Is DNA and Next-Generation Sequencing?
- Basics of DNA sequencing
- Advanced DNA sequencing and next-generation methods
- Next-generation sequencing technologies and terminology
- NGS and big data
- 2. Bioinformatics Interfaces for Next-Generation Sequencing
- Bioinformatics from the outside
- Pre-processing of NGS data
- 3. Next-Generation Sequencing Using a Reference Genome
- Alignment
- DNA variants
- RNA
- Epigenetics
- Metagenomics
- 4. Next-Generation Sequencing without a Reference Genome
- De novo genome assembly
- De novo RNA assembly
- 5. Next-Generation Sequencing Research and Technology
- Genome-wide association studies: An introduction
- The genome-wide association study
- Open-source integrative platforms
- 6. The Rest
- Contributing authors
