Chromatography
From LIMSWiki
Chromatography is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds (analytes or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."[1]
Chromatography has numerous methods associated with, dependent on the process-specific movement and type of mobile phase[1]:
Methods of chromatography | ||
---|---|---|
Mobility or equilibria | Phase | Chromatography type |
→ | Liquid | Tswett, paper, thin-layer |
→ | Gas | Gas |
→ | Supercritical medium | Supercritical fluid |
→ | Electrical flow | Electro |
↔ | Sorbent liquid | Hypersorption, countercurrent, denuder |
↓↑ | Adsorption | Liquid-solid (gel), gas-solid, supercritical fluid-solid |
↓↑ | Chemisorption | Ion exchange, affinity, complex-forming |
↓↑ | Absorption (partition) | Liquid-liquid, gas-liquid |
↓↑ | Physical field | Field-flow fractionation |
Equilibrium (isotherm) | Techniques, channel | Hyphenated mode |
Linear | Zone, column | One-dimensional |
Non-linear | Frontal, slot | Multidimensional |
Non-linear | Displacement, flat bed | Combined with spectral methods |
(Note: Arrows in the table indicate the movement of the analyte-containing mobile phase.)
See also
- Analyte
- Analytical chromatography
- Chromatogram
- Chromatograph
- Chromatography data management system (CDS)
- Fast protein liquid chromatography
- Gas chromatography
- Gel permeation chromatography
- High-performance liquid chromatography
- Ion chromatography
- Preparative high-performance liquid chromatography
- Supercritical fluid chromatography
- Ultra high-performance liquid chromatography
References
- ↑ 1.0 1.1 Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav (2011). "Chapter 1: Chromatography - A New Discipline of Science". Chromatography: A Science of Discovery. John Wiley & Sons. pp. 1–13. ISBN 1118060296. http://books.google.com/books?id=pKKoOCsytBMC.