Journal:Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L
| Full article title |
Development and validation of a fast gas chromatography–mass spectrometry method for the determination of cannabinoids in Cannabis sativa L |
|---|---|
| Journal | Journal of Food and Drug Analysis |
| Author(s) | Cardenia, Vladimiro; Toschi, Tullia G.; Scappini, Simona; Rubino, Rosamaria C.; Rodriguez-Estrada, Maria T. |
| Author affiliation(s) | University of Bologna, Enecta B.V. |
| Primary contact | Email: tullia dot gallinatoschi at unibo dot it |
| Year published | 2018 |
| Volume and issue | 26(4) |
| Page(s) | 1283–92 |
| DOI | 10.1016/j.jfda.2018.06.001 |
| ISSN | 1021-9498 |
| Distribution license | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
| Website | https://www.sciencedirect.com/science/article/pii/S1021949818301066 |
| Download | https://www.sciencedirect.com/science/article/pii/S1021949818301066/pdfft (PDF) |
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This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
A routine method for determining cannabinoids in Cannabis sativa L. inflorescence, based on fast gas chromatography coupled to mass spectrometry (Fast GC-MS), was developed and validated. To avoid the decarboxylation of the carboxyl group of cannabinoids, different derivatization approaches—i.e., silylation and esterification (diazomethane-mediated) reagents and solvents (pyridine or ethyl acetate)—were tested. The methylation significantly increased the signal-to-noise ratio of all carboxylic cannabinoids, except for cannabigerolic acid (CBGA). Since diazomethane is not commercially available, is considered a hazardous reactive, and requires one-day synthesis by specialized chemical staff, the process of silylation was used along the entire validation of a routine method. The method gave a fast (total analysis time < 7.0 min) and satisfactory resolution (R > 1.1), with a good repeatability (intraday < 8.38%; interday < 11.10%) and sensitivity (LOD < 11.20 ng/mL). The suitability of the fast GC-MS method for detection of cannabinoids in hemp inflorescences was tested; a good repeatability (intraday < 9.80%; interday < 8.63%), sensitivity (LOD < 58.89 ng/mg), and robustness (< 9.52%) was also obtained. In the analyzed samples, the main cannabinoid was cannabidiolic acid (CBDA; 5.19 ± 0.58 g/100 g), followed by cannabidiol (CBD; 1.56 ± 0.03 g/100 g) and CBGA (0.83 g/100 g). Δ9-tetrahydrocannabivarin (THCV) was present at trace levels. Therefore, the developed fast GC-MS method could be a valid, routine alternative for a fast, robust, and highly sensitive determination of the main cannabinoids present in hemp inflorescences.
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Notes
This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added. Everything else remains true to the original article, per the "NoDerivatives" portion of the distribution license.
