File:Fig1 Matielo Publications2018 6-4.png

Figure 1. Patterns of publication on Cannabis recovered from the Scopus platform using six different search terms. (a) Relative growth rate (RGR) of world’s scientific publications for each search argument. (b) Absolute number of publications for each search argument. Three important events related to the development of biotechnological methods are highlighted. First, in 1977, Fred Sanger described an enzyme-based methodology for DNA sequencing. Second, in 1989, Mullis and Faloona proposed the Polymerase Chain Reaction (PCR) methodology for in vitro DNA amplification. Third, in early 2005, the next-generation sequencing (NGS) was introduced by Life Science through the 454-pyrosequencing methodology.

{{{date}}}

Matielo, C.B.D.; Sarzi, D.S.; Justolin, B.; Lemos, R.P.M.; Camargo, F.A.O.; Stefenon, V.M.

See below.