Journal:Simultaneous quantification of 17 cannabinoids in cannabis inflorescence by liquid chromatography–mass spectrometry

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Full article title Simultaneous quantification of 17 cannabinoids in cannabis inflorescence by liquid chromatography–mass spectrometry
Journal Separations
Author(s) Hewavitharana, Amitha K.; Gloerfelt-Tarp, Francine; Nolan, Matthew; Barkla, Bronwyn J.; Purdy, Sarah; Kretzschmar, Tobias
Author affiliation(s) Southern Cross University, New South Wales Department of Primary Industries
Primary contact Email: a dot hewavitharana at pharmacy dot uq dot edu dot au
Year published 2022
Volume and issue 9(4)
Article # 85
DOI 10.3390/separations9040085
ISSN 2297-8739
Distribution license Creative Commons Attribution 4.0 International
Website https://www.mdpi.com/2297-8739/9/4/85/htm
Download https://www.mdpi.com/2297-8739/9/4/85/pdf (PDF)

Abstract

With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand for accurate and reliable quantification for a growing number of them. Although recent methods are based on highly selective chromatographymass spectrometry technology, most are limited to a few cannabinoids, while relying on unnecessarily sophisticated and expensive ultra-high-performance liquid chromatography and tandem mass spectrometry. Here we report an optimized, simple extraction method followed by a reliable and simple high-performance liquid chromatography (HPLC) method for separation. The detection is performed using a time-of-flight mass spectrometer that is available in most natural products research laboratories. Due to the simplicity of instrumentation, and the robustness resulting from a high resolution in the chromatography of isobaric cannabinoids, the method is well-suited for routine phytocannabinoid analysis for a range of applications. The method was validated in terms of detection and quantification limits, repeatability, and recoveries for a total of 17 cannabinoids. Detection limits were in the range 11–520 pg when using a 1 µL sample injection volume, and the recovery percentages ranged from 85% to 108%. The validated method was subsequently applied to determine cannabinoid composition in the inflorescences of several medicinal Cannabis sativa varieties.

Keywords: cannabinoids, phytocannabinoids, LC-MS, Cannabis sativa, tetrahydrocannabinol, THC, cannabidiol, CBD

Introduction

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Notes

This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added.