Journal:Simultaneous quantification of 17 cannabinoids in cannabis inflorescence by liquid chromatography–mass spectrometry
| Full article title | Simultaneous quantification of 17 cannabinoids in cannabis inflorescence by liquid chromatography–mass spectrometry |
|---|---|
| Journal | Separations |
| Author(s) | Hewavitharana, Amitha K.; Gloerfelt-Tarp, Francine; Nolan, Matthew; Barkla, Bronwyn J.; Purdy, Sarah; Kretzschmar, Tobias |
| Author affiliation(s) | Southern Cross University, New South Wales Department of Primary Industries |
| Primary contact | Email: a dot hewavitharana at pharmacy dot uq dot edu dot au |
| Year published | 2022 |
| Volume and issue | 9(4) |
| Article # | 85 |
| DOI | 10.3390/separations9040085 |
| ISSN | 2297-8739 |
| Distribution license | Creative Commons Attribution 4.0 International |
| Website | https://www.mdpi.com/2297-8739/9/4/85/htm |
| Download | https://www.mdpi.com/2297-8739/9/4/85/pdf (PDF) |
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This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
With an increasing appreciation for the unique pharmacological properties associated with distinct, individual cannabinoids of Cannabis sativa, there is demand for accurate and reliable quantification for a growing number of them. Although recent methods are based on highly selective chromatography–mass spectrometry technology, most are limited to a few cannabinoids, while relying on unnecessarily sophisticated and expensive ultra-high-performance liquid chromatography and tandem mass spectrometry. Here we report an optimized, simple extraction method followed by a reliable and simple high-performance liquid chromatography (HPLC) method for separation. The detection is performed using a time-of-flight mass spectrometer that is available in most natural products research laboratories. Due to the simplicity of instrumentation, and the robustness resulting from a high resolution in the chromatography of isobaric cannabinoids, the method is well-suited for routine phytocannabinoid analysis for a range of applications. The method was validated in terms of detection and quantification limits, repeatability, and recoveries for a total of 17 cannabinoids. Detection limits were in the range 11–520 pg when using a 1 µL sample injection volume, and the recovery percentages ranged from 85% to 108%. The validated method was subsequently applied to determine cannabinoid composition in the inflorescences of several medicinal Cannabis sativa varieties.
Keywords: cannabinoids, phytocannabinoids, LC-MS, Cannabis sativa, tetrahydrocannabinol, THC, cannabidiol, CBD
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This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added.
