Nucleic acid methods
From LIMSWiki
For protein methods, see Protein methods.
Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
- Purification
- Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: Q-PCR
- high-throughput relative abundance DNA microarray
- high-throughput absolute abundance SAGE
- Size: Gel electrophoresis
- Synthesis
- De novo: Oligonucleotide synthesis
- Amplification: PCR
- Kinetics
- Other
- Nucleic acid simulations
- DNA sequencing
- Bisulfite sequencing
- Expression cloning
- Southern blot
- Northern blot
- Sucrose gradient centrifugation
- Radioactivity in biological research
- Lab-on-a-chip
- Nuclear run-on assay
- Fluorescent in situ hybridization
- several Bioinformatics methods, such as RNA structure prediction
References
- ↑ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ↑ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques 19 (3): 151–158. PMC 2563918. PMID 19137101. https://www.ncbi.nlm.nih.gov/pmc/articles/2563918.
