Journal:Analysis of phenolic compounds in commercial Cannabis sativa L. inflorescences using UHPLC-Q-Orbitrap HRMS
| Full article title | Analysis of phenolic compounds in commercial Cannabis sativa L. inflorescences using UHPLC-Q-Orbitrap HRMS |
|---|---|
| Journal | Molecules |
| Author(s) |
Izzo, Luana; Castaldo, Luigi; Narváez, Alfonso; Graziani, Giulia; Gaspari, Anna; Rodríguez-Carrasco, Yelko; Ritieni, Alberto |
| Author affiliation(s) | University of Naples "Federico II," University of Valencia |
| Primary contact | Email: luana dot izzo at unina dot it |
| Editors | Efferth, Thomas |
| Year published | 2020 |
| Volume and issue | 25(3) |
| Article # | 631 |
| DOI | 10.3390/molecules25030631 |
| ISSN | 1420-3049 |
| Distribution license | Creative Commons Attribution 4.0 International |
| Website | https://www.mdpi.com/1420-3049/25/3/631/htm |
| Download | https://www.mdpi.com/1420-3049/25/3/631/pdf (PDF) |
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This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
Industrial hemp (Cannabis sativa L., family Cannabaceae) contains a vast number of relevant bioactive organic compounds, namely polyphenols, including flavonoids, phenolic acids, phenol amides, and lignanamides, which are well known for their therapeutic properties. Nowadays, many polyphenol-containing products made from herbal extracts are marketed, claiming to have health-promoting effects. In this context, industrial hemp inflorescences may represent an innovative source of bioactive compounds to be used in nutraceutical formulations. The aim of this work was to provide a comprehensive analysis of the polyphenolic fraction contained in polar extracts of four different commercial cultivars—Kompolti, Tiborszallasi, Antal, and Selected Carmagnola (CS)—of hemp inflorescence through spectrophotometric (for total polyphenol content [TPC] and DPPH assays) and spectrometry measurement (using ultra high-performance liquid chromatography–quadrupole–orbitrap high-resolution mass spectrometry or UHPLC-Q-Orbitrap HRMS). Results highlighted a high content of cannflavin A and B in inflorescence samples, which appear to be cannabis-specific, with a mean value of 61.8 and 84.5 mg/kg, meaning a ten-to-hundred times increase compared to other parts of the plant. Among flavonols, quercetin-3-glucoside reached up to 285.9 mg/kg in the CS cultivar. Catechin and epicatechin were the most representative flavanols, with a mean concentration of 53.3 and 66.2 mg/kg, respectively, for all cultivars. TPC in inflorescence samples was quantified in the range of 10.51 to 52.58 mg GAE/g, and free radical-scavenging included in the range from 27.5 to 77.6 mmol trolox/kg. Therefore, C. sativa inflorescence could be considered as a potential novel source of polyphenols intended for nutraceutical formulations.
Keywords: Cannabis sativa L., polyphenols, UHPLC-Q-Orbitrap HRMS
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Notes
This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added.
