Journal:Enzyme immunoassay for measuring aflatoxin B1 in legal cannabis
| Full article title | Enzyme immunoassay for measuring aflatoxin B1 in legal cannabis |
|---|---|
| Journal | Toxins |
| Author(s) | Di Nardo, Fabio; Cavalera, Simone; Baggiani, Claudio; Ciarello, Matteo; Pazzi, Marco; Anfossi, Laura |
| Author affiliation(s) | University of Turin |
| Primary contact | Email: laura dot anfossi at unito dot it |
| Year published | 2020 |
| Volume and issue | 12(4) |
| Article # | 265 |
| DOI | 10.3390/toxins12040265 |
| ISSN | 2072-6651 |
| Distribution license | Creative Commons Attribution 4.0 International |
| Website | https://www.mdpi.com/2072-6651/12/4/265/htm |
| Download | https://www.mdpi.com/2072-6651/12/4/265/pdf (PDF) |
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This article should be considered a work in progress and incomplete. Consider this article incomplete until this notice is removed. |
Abstract
The diffusion of the legalization of cannabis for recreational, medicinal, and nutraceutical uses requires the development of adequate analytical methods to assure the safety and security of such products. In particular, aflatoxins are considered to pose a major risk for the health of cannabis consumers. Among analytical methods that allow for adequate monitoring of food safety, immunoassays play a major role thanks to their cost-effectiveness, high-throughput capacity, simplicity, and limited requirement for equipment and skilled operators. Therefore, a rapid and sensitive enzyme immunoassay has been adapted to measure the most hazardous aflatoxin B1 in cannabis products. The assay was acceptably accurate (recovery rate: 78–136%), reproducible (intra- and inter-assay means coefficients of variation 11.8% and 13.8%, respectively), and sensitive (limit of detection and range of quantification: 0.35 ng mL−1 and 0.4–2 ng mL−1, respectively corresponding to 7 ng g−1 and 8–40 ng g−1 in the plant), while providing results which agreed with a high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) method for the direct analysis of aflatoxin B1 in cannabis inflorescence and leaves. In addition, the carcinogenic aflatoxin B1 was detected in 50% of the cannabis products analyzed (14 samples collected from small retails) at levels exceeding those admitted by the European Union in commodities intended for direct human consumption, thus envisaging the need for effective surveillance of aflatoxin contamination in legal cannabis.
Keywords: mycotoxins, food safety, medicinal herbs, competitive immunoassay
Introduction
Cannabis sativa is a plant of the Cannabaceae family and is well-known for its content of biologically active chemical compounds, among which are the major compounds Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD). The flowering or fruiting tops of the Cannabis plant have been controlled in the United States under the Controlled Substances Act since 1970 under the drug class “Marihuana.”[1]
Cannabis products can be used for medicinal purposes (whether using the psychoactive constituent THC or the non-psychoactive constituent CBD, generally referred to as "medical cannabis"), in manufacturing ("industrial hemp"), and for non-medical intoxication ("recreational or psychoactive cannabis").[2] The number of active constituents found in cannabis and the variety of their effects have also suggested cannabis' potential use as a dietary supplement and nutraceutical.[1][3] According to the World Health Organization (WHO), recreational cannabis is the most widely used illicit drug and the most largely cultivated and trafficked worldwide.[4]
The therapeutic application of cannabis is increasing around the world.[5] For example, a medicine based on cannabis extract has been approved by the European Medicines Agency.[6] THC can be medically administered as capsules, mouth spray, or as flowers for making tea. And the U.S. Food and Drug Administration (FDA) has approved one cannabis-derived and three cannabis-related drug products.[7]
References
- ↑ 1.0 1.1 U.S. Food and Drug Administration. "FDA Regulation of Cannabis and Cannabis-Derived Products, Including Cannabidiol (CBD)". U.S. Food and Drug Administration. https://www.fda.gov/news-events/public-health-focus/fda-regulation-cannabis-and-cannabis-derived-products-including-cannabidiol-cbd. Retrieved 10 July 2019.
- ↑ Mead, A. (2019). "Legal and Regulatory Issues Governing Cannabis and Cannabis-Derived Products in the United States". Frontiers in Plant Science 10: 697. doi:10.3389/fpls.2019.00697. PMC PMC6590107. PMID 31263468. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6590107.
- ↑ Hartsel, J.A.; Eades, J.; Hickory, B.; Makriyannis, A. (2016). "Chapter 53: Cannabis sativa and Hemp". In Gupta, R.C.. Nutraceuticals: Efficacy, Safety and Toxicity. Academic Press. pp. 735–754. ISBN 9780128021477.
- ↑ World Health Organization. "Cannabis". Management of substance abuse. World Health Organization. https://www.who.int/substance_abuse/facts/cannabis/en/. Retrieved 20 April 2020.
- ↑ Bridgeman, M.B.; Abazia, D.T. (2017). "Medicinal Cannabis: History, Pharmacology, And Implications for the Acute Care Setting". P & T 42 (3): 180–88. PMC PMC5312634. PMID 28250701. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312634.
- ↑ European Monitoring Centre for Drugs and Drug Addiction (December 2018). "Medical use of cannabis and cannabinoids: Questions and answers for policymaking". EMCDDA. doi:0.2810/979004. https://www.emcdda.europa.eu/publications/rapid-communications/medical-use-of-cannabis-and-cannabinoids-questions-and-answers-for-policymaking_en. Retrieved 04 November 2019.
- ↑ Corroon, J.; Kight, R. (2018). "Regulatory Status of Cannabidiol in the United States: A Perspective". Cannabis and Cannabinoid Research 3 (1): 190-194. doi:10.1089/can.2018.0030. PMC PMC6154432. PMID 30283822. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154432.
Notes
This presentation is faithful to the original, with only a few minor changes to presentation. Some grammar and punctuation was cleaned up to improve readability. In some cases important information was missing from the references, and that information was added. In the original article, citations 1 and 4 are duplicates; that duplication was removed for this version.
