File:Fig4 Calabria BMCBioinformatics2015 16-Suppl9.jpg

Figure 4: LAM-PCR workflow and the corresponding adLIMS automated process. The LAM-PCR procedure is reported as diagram (A) in which from a selected sample, different linear amplifications may be processed followed then by a first and a second exponential PCR amplification. The procedure is developed in adLIMS starting from the experiment set-up (B) in which a user fills the form with the data relative to the experiment such as "Name", number of LAM-PCRs (N-LAM), "LAM ID" and "date". Then adLIMS creates a number of entries as reported in the field "N-LAM", for the subsequent step "LAM-PCR linear" (C), that will be then processed and expanded in the "LAM-PCR 1st exp" step (D) with details such as "Name", "Plate barcode" and "Enzyme". The last step is the "LAM-PCR 2nd exp" (E), corresponding to the second exponential amplification, in which the user completes additional data of the experiment ("concentration" and "quality").

Calabria, Andrea; Spinozzi, Giulio; Benedicenti, Fabrizio; Tenderini, Erika; Montini, Eugenio (2015). "adLIMS: A customized open source software that allows bridging clinical and basic molecular research studies". BMC Bioinformatics 16 (Suppl 9): S5. doi:10.1186/1471-2105-16-S9-S5. ISSN 1471-2105. http://www.biomedcentral.com/1471-2105/16/S9/S5. 

2015

Calabria, Andrea; Spinozzi, Giulio; Benedicenti, Fabrizio; Tenderini, Erika; Montini, Eugenio

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