Difference between revisions of "Nucleic acid methods"
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*Amplification: [[Polymerase chain reaction|PCR]] | *Amplification: [[Polymerase chain reaction|PCR]] | ||
;Kinetics | ;Kinetics | ||
*[[Dual polarization interferometry]]<ref>{{ | *[[Dual polarization interferometry]]<ref>{{Cite journal|last1 = Berney |first1 = H. |last2 = Oliver | first2 = K. |doi = 10.1016/j.bios.2004.12.024 |title = Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation |journal = Biosensors and Bioelectronics |volume = 21 |issue = 4 |pages = 618–626 |year = 2005 |pmid = 16202875|pmc = }}</ref> | ||
*[[QCM-D]]<ref>{{cite journal|last=Dixon|first=Matthew C.|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions|journal=Journal of Biomolecular Techniques|year=2008|month=July|volume=19|issue=3|pages=151–158|pmid=19137101|pmc=2563918}}</ref> | *[[QCM-D]]<ref>{{cite journal|last=Dixon|first=Matthew C.|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions|journal=Journal of Biomolecular Techniques|year=2008|month=July|volume=19|issue=3|pages=151–158|pmid=19137101|pmc=2563918}}</ref> | ||
Revision as of 21:43, 26 October 2011
For protein methods, see Protein methods.
Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
- Purification
- Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: Q-PCR
- high-throughput relative abundance DNA microarray
- high-throughput absolute abundance SAGE
- Size: Gel electrophoresis
- Synthesis
- De novo: Oligonucleotide synthesis
- Amplification: PCR
- Kinetics
- Other
- Nucleic acid simulations
- DNA sequencing
- Bisulfite sequencing
- Expression cloning
- Southern blot
- Northern blot
- Sucrose gradient centrifugation
- Radioactivity in biological research
- Lab-on-a-chip
- Nuclear run-on assay
- Fluorescent in situ hybridization
- several Bioinformatics methods, such as RNA structure prediction
References
- ↑ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ↑ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques 19 (3): 151–158. PMC 2563918. PMID 19137101. https://www.ncbi.nlm.nih.gov/pmc/articles/2563918.
