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==Summary== | |||
{{Information | |||
|Description='''Fig. 1''' Male plant sex marker analysis by qPCR and sequencing. '''A''' Box plot analysis of second derivative cycle quotient values for MADC2 and tissue control for 8,200 samples from SexID qPCR results. '''B''' Genetic alignment of Steep Hill TaqMan probe for male identification from targeted sequencing of 45 males. '''C''' Representative amplification plots for Steep Hill sex determination assay (top: MADC2 amplification plot) (bottom: tissue control amplification plot). '''D''' Genetic alignment of canonical MADC2 391-bp cloned fragment from Mandolino aligned to queried IDs in PacBio sequence from Pineapple Banana Bubba Kush. | |||
|Source={{cite journal |title=High-throughput methods to identify male ''Cannabis sativa'' using various genotyping methods |journal=Journal of Cannabis Research |author=Torres, A.; Pauli, C.; Givens, R.; Argyris, J.; Allen, K.; Monfort, A.; Gaudino, R.J. |volume=4 |at=57 |year=2022 |doi=10.1186/s42238-022-00164-7}} | |||
|Author=Torres, A.; Pauli, C.; Givens, R.; Argyris, J.; Allen, K.; Monfort, A.; Gaudino, R.J. | |||
|Date=2022 | |||
|Permission=[https://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International] | |||
}} | |||
== Licensing == | == Licensing == | ||
{{cc-by-4.0}} | {{cc-by-4.0}} |
Revision as of 20:04, 6 March 2023
Summary
Description |
Fig. 1 Male plant sex marker analysis by qPCR and sequencing. A Box plot analysis of second derivative cycle quotient values for MADC2 and tissue control for 8,200 samples from SexID qPCR results. B Genetic alignment of Steep Hill TaqMan probe for male identification from targeted sequencing of 45 males. C Representative amplification plots for Steep Hill sex determination assay (top: MADC2 amplification plot) (bottom: tissue control amplification plot). D Genetic alignment of canonical MADC2 391-bp cloned fragment from Mandolino aligned to queried IDs in PacBio sequence from Pineapple Banana Bubba Kush. |
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Source |
Torres, A.; Pauli, C.; Givens, R.; Argyris, J.; Allen, K.; Monfort, A.; Gaudino, R.J. (2022). "High-throughput methods to identify male Cannabis sativa using various genotyping methods". Journal of Cannabis Research 4: 57. doi:10.1186/s42238-022-00164-7. |
Date |
2022 |
Author |
Torres, A.; Pauli, C.; Givens, R.; Argyris, J.; Allen, K.; Monfort, A.; Gaudino, R.J. |
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This work is licensed under the Creative Commons Attribution 4.0 License. |
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