Difference between revisions of "Serial dilution"
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This article is a direct transclusion of [https://en.wikipedia.org/wiki/Serial_dilution the Wikipedia article] and therefore may not meet the same editing standards as LIMSwiki. | |||
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Latest revision as of 20:20, 20 September 2022
A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion. A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M ... Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (100.5-fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (100.25-fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics.
In biology and medicine
In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results.[1] Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators.[2]
In homeopathy
Serial dilution is one of the core foundational practices of homeopathy, with "succussion", or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.[3][4]
See also
References
- ^ K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. 69. ISBN 81-224-1494-X
- ^ Booth, C.; et al. (2006). Extremophiles. Methods in microbiology 35. Academic Press. p. 543. ISBN 978-0-12-521536-7.
- ^ Weissmann, Gerald (2006). "Homeopathy: Holmes, Hogwarts, and the Prince of Wales". The FASEB Journal. 20 (11): 1755–1758. doi:10.1096/fj.06-0901ufm. PMID 16940145. S2CID 9305843. Retrieved 2008-02-01.
- ^ Ernst, Edzard (November 2005). "Is homeopathy a clinically valuable approach?". Trends in Pharmacological Sciences. 26 (11): 547–548. CiteSeerX 10.1.1.385.5505. doi:10.1016/j.tips.2005.09.003. PMID 16165225.
- Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams & Wilkins, 2004, p. 24. ISBN 0-7817-4611-6.
External links
- How to Make Simple Solutions and Dilutions, Bates College
Notes
This article is a direct transclusion of the Wikipedia article and therefore may not meet the same editing standards as LIMSwiki.
