Difference between revisions of "Confocal microscopy"
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'''Confocal microscopy''' is an optical imaging technique used to increase optical resolution and contrast of a [[micrograph]] by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane.<ref name=Pawley_2006>{{cite book |author=Pawley JB (editor) |title=Handbook of Biological Confocal Microscopy |publisher=Springer |location=Berlin |year=2006 |edition = 3rd |isbn=038725921X}}</ref> It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection, and materials science. | |||
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Revision as of 17:09, 18 September 2022
Confocal microscopy is an optical imaging technique used to increase optical resolution and contrast of a micrograph by using point illumination and a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane.[1] It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection, and materials science.
References
- ↑ Pawley JB (editor) (2006). Handbook of Biological Confocal Microscopy (3rd ed.). Berlin: Springer. ISBN 038725921X.
