Nucleic acid methods - Revision history

Shawndouglas: Cat

2022-09-20T18:30:41Z

Cat

← Older revision		Revision as of 18:30, 20 September 2022
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	[[Category:~~Biotechnology~~]]		[[Category:Genomics]]

Shawndouglas: Updated reference.

2011-10-26T21:43:37Z

Updated reference.

← Older revision		Revision as of 21:43, 26 October 2011
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	*Amplification: [[Polymerase chain reaction\|PCR]]		*Amplification: [[Polymerase chain reaction\|PCR]]
	;Kinetics		;Kinetics
	*[[Dual polarization interferometry]]<ref>{{~~cite~~ doi\|10.1016/j.bios.2004.12.024}}</ref>		*[[Dual polarization interferometry]]<ref>{{Cite journal\|last1 = Berney \|first1 = H. \|last2 = Oliver \| first2 = K. \|doi = 10.1016/j.bios.2004.12.024 \|title = Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation \|journal = Biosensors and Bioelectronics \|volume = 21 \|issue = 4 \|pages = 618–626 \|year = 2005 \|pmid = 16202875\|pmc = }}</ref>
	*[[QCM-D]]<ref>{{cite journal\|last=Dixon\|first=Matthew C.\|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions\|journal=Journal of Biomolecular Techniques\|year=2008\|month=July\|volume=19\|issue=3\|pages=151–158\|pmid=19137101\|pmc=2563918}}</ref>		*[[QCM-D]]<ref>{{cite journal\|last=Dixon\|first=Matthew C.\|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions\|journal=Journal of Biomolecular Techniques\|year=2008\|month=July\|volume=19\|issue=3\|pages=151–158\|pmid=19137101\|pmc=2563918}}</ref>

Shawndouglas: Created article.

2011-10-26T21:39:03Z

Created article.

New page

{{for|protein methods|Protein methods}}
'''Nucleic acid methods''' are the techniques used to study [[nucleic acid]]s ([[DNA]] and [[RNA]]).

;Purification
*[[Guanidinium thiocyanate-phenol-chloroform extraction|Phenol-chloroform extraction]]
*[[minicolumn purification]]
*[[RNA extraction]]
;Quantification
*Abundance in weight: [[Quantification of nucleic acids|spectroscopic quantification]]
*Absolute abundance in number: [[Real-time polymerase chain reaction|Q-PCR]]
*high-throughput relative abundance [[DNA microarray]]
*high-throughput absolute abundance [[serial analysis of gene expression|SAGE]]
*Size: [[Gel electrophoresis]]
;Synthesis
*De novo: [[Oligonucleotide synthesis]]
*Amplification: [[Polymerase chain reaction|PCR]]
;Kinetics
*[[Dual polarization interferometry]]<ref>{{cite doi|10.1016/j.bios.2004.12.024}}</ref>
*[[QCM-D]]<ref>{{cite journal|last=Dixon|first=Matthew C.|title=Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions|journal=Journal of Biomolecular Techniques|year=2008|month=July|volume=19|issue=3|pages=151–158|pmid=19137101|pmc=2563918}}</ref>

;Other
*[[List of nucleic acid simulation software|Nucleic acid simulations]]
*[[DNA sequencing]]
*[[Bisulfite sequencing]]
*[[Expression cloning]]
*[[Southern blot]]
*[[Northern blot]]
*[[Sucrose gradient centrifugation]]
*[[Radioactivity in biological research]]
*[[Lab-on-a-chip]]
*[[Nuclear run-on|Nuclear run-on assay]]
*[[Fluorescent in situ hybridization]]
*several [[Bioinformatics]] methods, such as [[List of RNA structure prediction software|RNA structure prediction]]

== References ==
{{reflist}}

== External links ==
* [http://www.genome.ou.edu/protocol_book/protocol_index.html Protocols for Recombinant DNA Isolation, Cloning, and Sequencing]

[[Category:Biotechnology]]