Chromatography - Revision history

Shawndouglas: Updated to transclusion

2020-03-10T22:44:27Z

Updated to transclusion

← Older revision		Revision as of 22:44, 10 March 2020
Line 1:		Line 1:
	'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{~~cite book \|url=http~~:~~//books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1~~: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref> Components requiring separation join with the mobile phase — a gas or a liquid, for example — and pass through an immobile or stationary phase — a liquid or a solid. The components sorb or accumulate either in the stationary phase or at the border of where the phases interface, moving at different speeds based on sorptivity and forming a regular distribution.<ref name=~~"ChromSharma">{{cite book \|url=http://books.google.com/books?id~~=~~HkLq-fCAxg0C \|chapter~~=~~Chapter 1: Chromatography \|title~~=~~Chromatography \|author=Sharma, B.K. \|publisher=Krishna Prakashan Media \|year=2007 \|pages=C1–C20 \|isbn=8185842795}}</ref>~~ This ~~distribution~~ is ~~measured with the help of a chromatograph, spectrophotometer, mass spectrometer, and/or other detector and output as a chromatogram, typically in graph form as a function of elution time.~~		{{wikipedia::Chromatography}}
			==Notes==
	~~==Methods==~~		This article is a direct transclusion of [https://en.wikipedia.org/wiki/Chromatography the Wikipedia article] and therefore may not meet the same editing standards as LIMSwiki.

	Chromatographic separations were originally confined to colored plant pigments and dyes, but they quickly expanded to include colorless substances. Today, chromatographic methodology has expanded greatly with technological and scientific innovation, with a ~~method for almost any type of analyte or particulate matter in the most minute of quantity.<ref name="ChromSharma" />~~

	~~Chromatography has numerous associated methods, dependent on the process-specific movement and type~~ of ~~mobile phase<ref name="ChromSciDiscov" />:~~

	~~{\| class="wikitable" border="1" cellpadding="5" cellspacing="0" width="700"~~
	\|-
	~~! colspan="3"\| Methods of chromatography~~
	\|-
	~~! style="color:brown; background-color:#ffffee;"\| Mobility or equilibria~~
	~~! style="color:brown; background-color:#ffffee;"\| Phase~~
	~~! style="color:brown; background-color:#ffffee;"\| Chromatography type~~
	\|-
	~~\| style="text-align:center;" \|'''→'''~~
	~~\| style="background-color:white;" \| Liquid~~
	~~\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]]), [[Paper chromatography\|paper]], [~~[~~Thin-layer chromatography\|thin-layer]]~~
	\|-
	~~\| style="text-align:center;" \|'''→'''~~
	~~\| style="background-color:white;" \| Gas~~
	~~\| style="background-color:white;" \| [[Gas chromatography\|Gas]]~~
	\|-
	~~\| style="text-align:center;" \|'''→'''~~
	~~\| style="background-color:white;" \| Supercritical medium~~
	~~\| style="background-color:white;" \| [[Supercritical fluid chromatography\|Supercritical fluid]]~~
	\|-
	~~\| style="text-align:center;" \|'''→'''~~
	~~\| style="background-color:white;" \| Electrical flow~~
	~~\| style="background-color:white;" \| [[Electrochromatography\|Electro]]~~
	\|-
	~~\| style="text-align:center;" \|'''↔'''~~
	~~\| style="background-color:white;" \| Sorbent liquid~~
	~~\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], [[Denuder\|denuder]]~~
	\|-
	~~\| style="text-align:center;" \|'''↓↑'''~~
	~~\| style="background-color:white;" \| Adsorption~~
	~~\| style="background-color:white;" \| Liquid-solid ([[Gel permeation chromatography\|gel]]), gas-solid, supercritical fluid-solid~~
	\|-
	~~\| style="text-align:center;" \|'''↓↑'''~~
	~~\| style="background-color:white;" \| Chemisorption~~
	~~\| style="background-color:white;" \| [[Ion chromatography\|Ion exchange]], [[Affinity chromatography\|affinity]], complex-forming~~
	\|-
	~~\| style="text-align:center;" \|'''↓↑'''~~
	~~\| style="background-color:white;" \| Absorption (partition)~~
	~~\| style="background-color:white;" \| Liquid-liquid, gas-liquid~~
	\|-
	~~\| style="text-align:center;" \|'''↓↑'''~~
	~~\| style="background-color:white;" \| Physical field~~
	~~\| style="background-color:white;" \| [[Field-flow fractionation]]~~
	\|-
	~~\| style="text-align:center;" \|'''Equilibrium (isotherm)'''~~
	~~\| style="background-color:white;" \| Techniques, channel~~
	~~\| style="background-color:white;" \| Hyphenated techniques~~
	\|-
	~~\| style="text-align:center;" \|'''Linear'''~~
	~~\| style="background-color:white;" \| Zone, column~~
	~~\| style="background-color:white;" \| One-dimensional~~
	\|-
	~~\| style="text-align:center;" \|'''Non-linear'''~~
	~~\| style="background-color:white;" \| Frontal, slot~~
	~~\| style="background-color:white;" \| Multidimensional~~
	\|-
	~~\| style="text-align:center;" \|'''Non-linear'''~~
	~~\| style="background-color:white;" \| Displacement, flat bed~~
	~~\| style="background-color:white;" \| Combined with spectral methods~~
	\|-
	\|}
	~~('''Note''': Arrows in the table indicate the movement of the analyte-containing mobile phase.)~~

	~~==See also==~~

	* [[Analytical chromatography]]
	* [[Chromatography data system]]
	* [[Fast protein liquid chromatography]]
	* [[Gas chromatography]]
	* [[Gel permeation chromatography]]
	* [[High-performance liquid chromatography]]
	* [[Ion chromatography]]
	* [[Preparative high-performance liquid chromatography]]
	* [[Supercritical fluid chromatography]]
	* [[Ultra high-performance liquid chromatography]]

	~~==Further reading==~~

	* {{cite book \|url=http://~~books.google~~.~~com/books?id=tdrFTHQ3e64C \|chapter=75 Years of Chromatography: A Historical Dialogue \|title=Journal of Chromatography Library - Volume 17 \|editor=Ettre, Leslie S~~.~~; Zlatkis, Albert \|publisher=Elsevier \|year=1979 \|pages=501 \|isbn=0080858171}}~~
	* {{cite book \|url=http://~~books.google.com~~/~~books?id=pKKoOCsytBMC \|title=~~Chromatography~~: A Science of Discovery \|editor=Wixom, Robert L.; Gehrke, Charles W~~. ~~\|publisher=John Wiley & Sons \|year=2011 \|pages=410 \|isbn=1118060296}}~~

	~~== References ==~~
	~~<references />~~

	<!---Place all category tags here-->		<!---Place all category tags here-->
			[[Category:Articles transcluded from other wikis]]
	[[Category:Chromatography]]		[[Category:Chromatography]]
	~~[[Category:Scientific techniques]]~~

Shawndouglas: /* See also */ CDMS to CDS

2019-04-18T20:38:23Z

Shawndouglas: /* References */ Added cat

2014-08-06T17:30:00Z

References: Added cat

← Older revision		Revision as of 17:30, 6 August 2014
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	<!---Place all category tags here-->		<!---Place all category tags here-->
	[[Category:Chromatography]]		[[Category:Chromatography]]
			[[Category:Scientific techniques]]

Shawndouglas: /* Methods */ Updated table.

2013-10-14T23:27:51Z

Methods: Updated table.

Shawndouglas: /* Methods */ Minor fixes

2013-10-14T21:46:07Z

Methods: Minor fixes

← Older revision		Revision as of 21:46, 14 October 2013
Line 17:		Line 17:
	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
	\| style="background-color:white;" \| Liquid		\| style="background-color:white;" \| Liquid
	\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]], [[Paper chromatography\|paper]], [[Thin-layer chromatography\|thin-layer]]		\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]]), [[Paper chromatography\|paper]], [[Thin-layer chromatography\|thin-layer]]
	\|-		\|-
	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
Line 33:		Line 33:
	\| style="text-align:center;" \|'''↔'''		\| style="text-align:center;" \|'''↔'''
	\| style="background-color:white;" \| Sorbent liquid		\| style="background-color:white;" \| Sorbent liquid
	\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], denuder		\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], [[Denuder\|denuder]]
	\|-		\|-
	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''

Shawndouglas: Minor updates

2013-10-14T21:45:29Z

Minor updates

Shawndouglas: Added more content.

2013-10-14T21:08:45Z

Added more content.

← Older revision		Revision as of 21:08, 14 October 2013
Line 1:		Line 1:
	'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref>		'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref> Components requiring separation join with the mobile phase — a gas or a liquid, for example — and pass through an immobile or stationary phase — a liquid or a solid. The components sorb or accumulate either in the stationary phase or at the border of where the phases interface, moving at different speeds based on sorptivity and forming a regular distribution.<ref name="ChromSharma">{{cite book \|url=http://books.google.com/books?id=HkLq-fCAxg0C \|chapter=Chapter 1: Chromatography \|title=Chromatography \|author=Sharma, B.K. \|publisher=Krishna Prakashan Media \|year=2007 \|pages=C1–C20 \|isbn=8185842795}}</ref> This distribution is measured with the help of a chromatograph, spectrophotometer, mass spectrometer, and/or other detector and output as a chromatogram.

	Chromatography has numerous methods ~~associated with~~, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:		==Methods==

			Chromatographic separations were originally confined to colored plant pigments and dyes, but they quickly expanded to include colorless substances. Today, chromatographic methodology has expanded greatly with technological and scientific innovation, with a method for almost any type of analyte or particulate matter in the most minute of quantity.<ref name="ChromSharma" />

			Chromatography has numerous associated methods, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:

	{\| class="wikitable collapsible" border="1" cellpadding="5" cellspacing="0" width="700"		{\| class="wikitable collapsible" border="1" cellpadding="5" cellspacing="0" width="700"
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	('''Note''': Arrows in the table indicate the movement of the analyte-containing mobile phase.)		('''Note''': Arrows in the table indicate the movement of the analyte-containing mobile phase.)

			==See also==

	~~== See also ==~~

	* [[Analyte]]
	* [[Analytical chromatography]]		* [[Analytical chromatography]]
	* [[Chromatogram]]		* [[Chromatography data management system]]
	* [[Chromatograph]]
	* [[Chromatography data management system]] ~~(CDS)~~
	* [[Fast protein liquid chromatography]]		* [[Fast protein liquid chromatography]]
	* [[Gas chromatography]]		* [[Gas chromatography]]
Line 82:		Line 82:
	* [[Supercritical fluid chromatography]]		* [[Supercritical fluid chromatography]]
	* [[Ultra high-performance liquid chromatography]]		* [[Ultra high-performance liquid chromatography]]

			==Further reading==

			* {{cite book \|url=http://books.google.com/books?id=tdrFTHQ3e64C \|chapter=75 Years of Chromatography: A Historical Dialogue \|title=Journal of Chromatography Library - Volume 17 \|editor=Ettre, Leslie S.; Zlatkis, Albert \|publisher=Elsevier \|year=1979 \|pages=501 \|isbn=0080858171}}
			* {{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|title=Chromatography: A Science of Discovery \|editor=Wixom, Robert L.; Gehrke, Charles W. \|publisher=John Wiley & Sons \|year=2011 \|pages=410 \|isbn=1118060296}}

	== References ==		== References ==

Shawndouglas: Updated

2013-10-14T19:33:34Z

Updated

← Older revision		Revision as of 19:33, 14 October 2013
Line 1:		Line 1:
	~~Set~~ of [[~~laboratory~~]] ~~techniques for~~ the ~~separation of mixtures involving passing a mixture dissolved~~ in a "mobile phase~~" through~~ a stationary phase ~~which separates the~~ [[~~analyte~~]] ~~to be measured from other molecules~~ in the ~~mixture based on differential partitioning between~~ the mobile ~~and stationary phases~~.		'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref>

			Chromatography has numerous methods associated with, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:

			{\| class="wikitable collapsible" border="1" cellpadding="5" cellspacing="0" width="700"
			\|-
			! colspan="3"\| Methods of chromatography
			\|-
			! style="color:brown; background-color:#ffffee;"\| Mobility or equilibria
			! style="color:brown; background-color:#ffffee;"\| Phase
			! style="color:brown; background-color:#ffffee;"\| Chromatography type
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Liquid
			\| style="background-color:white;" \| Tswett, paper, thin-layer
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Gas
			\| style="background-color:white;" \| [[Gas chromatography\|Gas]]
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Supercritical medium
			\| style="background-color:white;" \| [[Supercritical fluid chromatography\|Supercritical fluid]]
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Electrical flow
			\| style="background-color:white;" \| Electro
			\|-
			\| style="text-align:center;" \|'''↔'''
			\| style="background-color:white;" \| Sorbent liquid
			\| style="background-color:white;" \| Hypersorption, countercurrent, denuder
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Adsorption
			\| style="background-color:white;" \| Liquid-solid ([[Gel permeation chromatography\|gel]]), gas-solid, supercritical fluid-solid
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Chemisorption
			\| style="background-color:white;" \| [[Ion chromatography\|Ion exchange]], affinity, complex-forming
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Absorption (partition)
			\| style="background-color:white;" \| Liquid-liquid, gas-liquid
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Physical field
			\| style="background-color:white;" \| Field-flow fractionation
			\|-
			\| style="text-align:center;" \|'''Equilibrium (isotherm)'''
			\| style="background-color:white;" \| Techniques, channel
			\| style="background-color:white;" \| Hyphenated mode
			\|-
			\| style="text-align:center;" \|'''Linear'''
			\| style="background-color:white;" \| Zone, column
			\| style="background-color:white;" \| One-dimensional
			\|-
			\| style="text-align:center;" \|'''Non-linear'''
			\| style="background-color:white;" \| Frontal, slot
			\| style="background-color:white;" \| Multidimensional
			\|-
			\| style="text-align:center;" \|'''Non-linear'''
			\| style="background-color:white;" \| Displacement, flat bed
			\| style="background-color:white;" \| Combined with spectral methods
			\|-
			\|}
			('''Note''': Arrows in the table indicate the movement of the analyte-containing mobile phase.)


	== See also ==		== See also ==
Line 16:		Line 82:
	* [[Supercritical fluid chromatography]]		* [[Supercritical fluid chromatography]]
	* [[Ultra high-performance liquid chromatography]]		* [[Ultra high-performance liquid chromatography]]

			== References ==
			<references />

			<!---Place all category tags here-->
			[[Category:Chromatography]]

Shawndouglas: Added more links to See also

2011-05-29T00:39:36Z

Added more links to See also

← Older revision		Revision as of 00:39, 29 May 2011
Line 8:		Line 8:
	* [[Chromatograph]]		* [[Chromatograph]]
	* [[Chromatography data management system]] (CDS)		* [[Chromatography data management system]] (CDS)
			* [[Fast protein liquid chromatography]]
	* [[Gas chromatography]]		* [[Gas chromatography]]
			* [[Gel permeation chromatography]]
	* [[High-performance liquid chromatography]]		* [[High-performance liquid chromatography]]
			* [[Ion chromatography]]
			* [[Preparative high-performance liquid chromatography]]
			* [[Supercritical fluid chromatography]]
			* [[Ultra high-performance liquid chromatography]]

Shawndouglas: Corrected link to HPLC

2011-05-28T23:21:17Z

Corrected link to HPLC

← Older revision		Revision as of 23:21, 28 May 2011
Line 9:		Line 9:
	* [[Chromatography data management system]] (CDS)		* [[Chromatography data management system]] (CDS)
	* [[Gas chromatography]]		* [[Gas chromatography]]
	* [[High performance liquid chromatography]]		* [[High-performance liquid chromatography]]

← Older revision		Revision as of 21:46, 14 October 2013
Line 17:		Line 17:
	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
	\| style="background-color:white;" \| Liquid		\| style="background-color:white;" \| Liquid
	\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]], [[Paper chromatography\|paper]], [[Thin-layer chromatography\|thin-layer]]		\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]]), [[Paper chromatography\|paper]], [[Thin-layer chromatography\|thin-layer]]
	\|-		\|-
	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
Line 33:		Line 33:
	\| style="text-align:center;" \|'''↔'''		\| style="text-align:center;" \|'''↔'''
	\| style="background-color:white;" \| Sorbent liquid		\| style="background-color:white;" \| Sorbent liquid
	\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], denuder		\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], [[Denuder\|denuder]]
	\|-		\|-
	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''

← Older revision		Revision as of 19:33, 14 October 2013
Line 1:		Line 1:
	~~Set~~ of [[~~laboratory~~]] ~~techniques for~~ the ~~separation of mixtures involving passing a mixture dissolved~~ in a "mobile phase~~" through~~ a stationary phase ~~which separates the~~ [[~~analyte~~]] ~~to be measured from other molecules~~ in the ~~mixture based on differential partitioning between~~ the mobile ~~and stationary phases~~.		'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref>

			Chromatography has numerous methods associated with, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:

			{\| class="wikitable collapsible" border="1" cellpadding="5" cellspacing="0" width="700"
			\|-
			! colspan="3"\| Methods of chromatography
			\|-
			! style="color:brown; background-color:#ffffee;"\| Mobility or equilibria
			! style="color:brown; background-color:#ffffee;"\| Phase
			! style="color:brown; background-color:#ffffee;"\| Chromatography type
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Liquid
			\| style="background-color:white;" \| Tswett, paper, thin-layer
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Gas
			\| style="background-color:white;" \| [[Gas chromatography\|Gas]]
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Supercritical medium
			\| style="background-color:white;" \| [[Supercritical fluid chromatography\|Supercritical fluid]]
			\|-
			\| style="text-align:center;" \|'''→'''
			\| style="background-color:white;" \| Electrical flow
			\| style="background-color:white;" \| Electro
			\|-
			\| style="text-align:center;" \|'''↔'''
			\| style="background-color:white;" \| Sorbent liquid
			\| style="background-color:white;" \| Hypersorption, countercurrent, denuder
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Adsorption
			\| style="background-color:white;" \| Liquid-solid ([[Gel permeation chromatography\|gel]]), gas-solid, supercritical fluid-solid
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Chemisorption
			\| style="background-color:white;" \| [[Ion chromatography\|Ion exchange]], affinity, complex-forming
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Absorption (partition)
			\| style="background-color:white;" \| Liquid-liquid, gas-liquid
			\|-
			\| style="text-align:center;" \|'''↓↑'''
			\| style="background-color:white;" \| Physical field
			\| style="background-color:white;" \| Field-flow fractionation
			\|-
			\| style="text-align:center;" \|'''Equilibrium (isotherm)'''
			\| style="background-color:white;" \| Techniques, channel
			\| style="background-color:white;" \| Hyphenated mode
			\|-
			\| style="text-align:center;" \|'''Linear'''
			\| style="background-color:white;" \| Zone, column
			\| style="background-color:white;" \| One-dimensional
			\|-
			\| style="text-align:center;" \|'''Non-linear'''
			\| style="background-color:white;" \| Frontal, slot
			\| style="background-color:white;" \| Multidimensional
			\|-
			\| style="text-align:center;" \|'''Non-linear'''
			\| style="background-color:white;" \| Displacement, flat bed
			\| style="background-color:white;" \| Combined with spectral methods
			\|-
			\|}
			('''Note''': Arrows in the table indicate the movement of the analyte-containing mobile phase.)


	== See also ==		== See also ==
Line 16:		Line 82:
	* [[Supercritical fluid chromatography]]		* [[Supercritical fluid chromatography]]
	* [[Ultra high-performance liquid chromatography]]		* [[Ultra high-performance liquid chromatography]]

			== References ==
			<references />

			<!---Place all category tags here-->
			[[Category:Chromatography]]

← Older revision		Revision as of 20:38, 18 April 2019
Line 73:		Line 73:

	* [[Analytical chromatography]]		* [[Analytical chromatography]]
	* [[Chromatography data ~~management~~ system]]		* [[Chromatography data system]]
	* [[Fast protein liquid chromatography]]		* [[Fast protein liquid chromatography]]
	* [[Gas chromatography]]		* [[Gas chromatography]]

← Older revision		Revision as of 23:27, 14 October 2013
Line 7:		Line 7:
	Chromatography has numerous associated methods, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:		Chromatography has numerous associated methods, dependent on the process-specific movement and type of mobile phase<ref name="ChromSciDiscov" />:

	{\| class="wikitable ~~collapsible~~" border="1" cellpadding="5" cellspacing="0" width="700"		{\| class="wikitable" border="1" cellpadding="5" cellspacing="0" width="700"
	\|-		\|-
	! colspan="3"\| Methods of chromatography		! colspan="3"\| Methods of chromatography
Line 41:		Line 41:
	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''
	\| style="background-color:white;" \| Chemisorption		\| style="background-color:white;" \| Chemisorption
	\| style="background-color:white;" \| [[Ion chromatography\|Ion exchange]], affinity, complex-forming		\| style="background-color:white;" \| [[Ion chromatography\|Ion exchange]], [[Affinity chromatography\|affinity]], complex-forming
	\|-		\|-
	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''
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	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''
	\| style="background-color:white;" \| Physical field		\| style="background-color:white;" \| Physical field
	\| style="background-color:white;" \| Field-flow fractionation		\| style="background-color:white;" \| [[Field-flow fractionation]]
	\|-		\|-
	\| style="text-align:center;" \|'''Equilibrium (isotherm)'''		\| style="text-align:center;" \|'''Equilibrium (isotherm)'''
	\| style="background-color:white;" \| Techniques, channel		\| style="background-color:white;" \| Techniques, channel
	\| style="background-color:white;" \| Hyphenated ~~mode~~		\| style="background-color:white;" \| Hyphenated techniques
	\|-		\|-
	\| style="text-align:center;" \|'''Linear'''		\| style="text-align:center;" \|'''Linear'''

← Older revision		Revision as of 21:45, 14 October 2013
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	'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref> Components requiring separation join with the mobile phase — a gas or a liquid, for example — and pass through an immobile or stationary phase — a liquid or a solid. The components sorb or accumulate either in the stationary phase or at the border of where the phases interface, moving at different speeds based on sorptivity and forming a regular distribution.<ref name="ChromSharma">{{cite book \|url=http://books.google.com/books?id=HkLq-fCAxg0C \|chapter=Chapter 1: Chromatography \|title=Chromatography \|author=Sharma, B.K. \|publisher=Krishna Prakashan Media \|year=2007 \|pages=C1–C20 \|isbn=8185842795}}</ref> This distribution is measured with the help of a chromatograph, spectrophotometer, mass spectrometer, and/or other detector and output as a chromatogram.		'''Chromatography''' is a continuously evolving scientific discipline or field "studying the formation, change, movement, and separation of multiple concentration zones of chemical compounds ([[Analyte\|analytes]] or particles) of the studied sample in a flow of mobile phase relative to selective influence of one or a number of solid/liquid stationary phases or sorbents."<ref name="ChromSciDiscov">{{cite book \|url=http://books.google.com/books?id=pKKoOCsytBMC \|chapter=Chapter 1: Chromatography - A New Discipline of Science \|title=Chromatography: A Science of Discovery \|author=Wixom, Robert L. (ed); Gehrke, Charles W. (ed.); Berezkin, Viktor G.; Janak, Jaroslav \|publisher=John Wiley & Sons \|year=2011 \|pages=1–13 \|isbn=1118060296}}</ref> Components requiring separation join with the mobile phase — a gas or a liquid, for example — and pass through an immobile or stationary phase — a liquid or a solid. The components sorb or accumulate either in the stationary phase or at the border of where the phases interface, moving at different speeds based on sorptivity and forming a regular distribution.<ref name="ChromSharma">{{cite book \|url=http://books.google.com/books?id=HkLq-fCAxg0C \|chapter=Chapter 1: Chromatography \|title=Chromatography \|author=Sharma, B.K. \|publisher=Krishna Prakashan Media \|year=2007 \|pages=C1–C20 \|isbn=8185842795}}</ref> This distribution is measured with the help of a chromatograph, spectrophotometer, mass spectrometer, and/or other detector and output as a chromatogram, typically in graph form as a function of elution time.

	==Methods==		==Methods==
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	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
	\| style="background-color:white;" \| Liquid		\| style="background-color:white;" \| Liquid
	\| style="background-color:white;" \| Tswett, paper, thin-layer		\| style="background-color:white;" \| Tswett ([[Column chromatography\|column]], [[Paper chromatography\|paper]], [[Thin-layer chromatography\|thin-layer]]
	\|-		\|-
	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
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	\| style="text-align:center;" \|'''→'''		\| style="text-align:center;" \|'''→'''
	\| style="background-color:white;" \| Electrical flow		\| style="background-color:white;" \| Electrical flow
	\| style="background-color:white;" \| Electro		\| style="background-color:white;" \| [[Electrochromatography\|Electro]]
	\|-		\|-
	\| style="text-align:center;" \|'''↔'''		\| style="text-align:center;" \|'''↔'''
	\| style="background-color:white;" \| Sorbent liquid		\| style="background-color:white;" \| Sorbent liquid
	\| style="background-color:white;" \| Hypersorption, countercurrent, denuder		\| style="background-color:white;" \| Hypersorption, [[Countercurrent chromatography\|countercurrent]], denuder
	\|-		\|-
	\| style="text-align:center;" \|'''↓↑'''		\| style="text-align:center;" \|'''↓↑'''